| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
29/12/2014 |
|
Data da última atualização: |
30/12/2014 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. |
|
Afiliação: |
CNPSO - estagiário; UENP; CNPSO - estagiário; UENP; CNPSO - estagiário; CNPSO - estagiário; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO. |
|
Título: |
Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Phakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. MenosPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. T... Mostrar Tudo |
|
Palavras-Chave: |
Ferrugem asiática da soja. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114606/1/Temporal-gene-expression-analysis-of-effector-candidates-of-Phakopsora-pachyrhizi-across-the-infection-cycle.pdf
|
|
Marc: |
LEADER 02492nam a2200193 a 4500
001 2003884
005 2014-12-30
008 2014 bl uuuu u00u1 u #d
100 1 $aPOLIZEL-PODANOSQUI, A. M.
245 $aTemporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions$c2014
520 $aPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust.
653 $aFerrugem asiática da soja
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aROCHA, C. S.
700 1 $aDARBEN, L. M.
700 1 $aLOPES-CAITAR, V. S.
700 1 $aAOYAOGI, L. N.
700 1 $aMARCELINO-GUIMARÃES, F. C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Florestas. |
|
Data corrente: |
04/01/2024 |
|
Data da última atualização: |
04/01/2024 |
|
Autoria: |
STANCK, J. A. T.; SANTOS, G. C. dos; TOLENTINO JÚNIOR, J. B.; GRAF JÚNIOR, A. L.; ITAKO, A. T. |
|
Afiliação: |
JULIANA APARECIDA TEIXEIRA STANCK, UNIVERSIDADE FEDERAL DE SANTA CATARINA; GABRIELA CAROLINA DOS SANTOS, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOÃO BATISTA TOLENTINO JÚNIOR, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ANDRÉ LUIZ GRAF JÚNIOR, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ADRIANA TERUMI ITAKO, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
|
Título: |
Sanidade e germinação de sementes de Mimosa scabrella submetidas ao tratamento com óleos essenciais. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 43, e202002147, p. 1-7, 2023 |
|
ISSN: |
1983-2605 |
|
DOI: |
https://doi.org/10.4336/2023.pfb.43e202002147 |
|
Idioma: |
Português |
|
Conteúdo: |
RESUMO. O objetivo desse estudo foi avaliar a sanidade e germinação de sementes de bracatinga (Mimosa scabrella Bentham) tratadas com óleos essenciais de cravo-da-índia (Syzygium aromaticum (L.) Merrill & Perry) e eucalipto citriodora (Corymbia citriodora (Hook.) KD Hill & LAS Johnson). O teste de germinação foi realizado em caixas gerbox e as avaliações foram realizadas aos 7 e 14 dias, contabilizando o número de plântulas normais, anormais, sementes mortas e duras. Para avaliar a fitotoxidez, as sementes foram tratadas em concentrações crescentes dos óleos essenciais (0, 250, 500 e 1000 ppm). Na avaliação do efeito inibitório dos óleos essenciais sobre a incidência de fungos presentes nas sementes, as mesmas foram tratadas com água destilada (controle negativo), hipoclorito de sódio 1% (controle positivo), óleo essencial de S. aromaticum 1000 ppm e óleo essencial de C. citriodora 1000 ppm. O delineamento utilizado foi inteiramente casualizado e os dados foram submetidos ao teste Z a 5% de probabilidade. Verificou-se a presença dos fungos Fusarium spp., Trichoderma spp., Pestalotiopsis spp. e Penicillium spp. nas sementes avaliadas. O óleo de cravo-da-índia apresentou potencial inibitório dos patógenos e não causou fitotoxidez nas sementes. ABSTRACT. Abstract -The objective of this study was to evaluate the health and germination of bracatinga seeds (M. scabrella Bentham) treated with essential oils of Syzygium aromaticum (L.) Merrill & Perry and Corymbia citriodora (Hook.) KD Hill & LAS Johnson. The germination test was carried out in gerbox boxes and evaluations were carried out at 7 and 14 days counting the number of normal and abnormal seedlings, dead and hard seeds. To assess phytotoxicity, seeds were treated with increasing concentration of essential oils (0, 250, 500 and 1000 ppm). To evaluate the inhibitory effect of essential oils on the incidence of microorganisms present in the seeds, they were treated with distilled water (negative control), sodium hypochlorite 1% (positive control), essential oil of S. aromaticum 1000 ppm and essential oil of C. citriodora 1000 ppm. The design was completely randomized, and the data were compared using the Z test at 5% probability. We verified the occurrence ofFusarium spp., Trichoderma spp., Pestalotiopsis spp. and Penicillium spp. pathogens at evaluated seeds. S. aromaticumessential oil showed potential to inhibit pathogens and did not cause phytotoxicity in M. scabrella seeds. MenosRESUMO. O objetivo desse estudo foi avaliar a sanidade e germinação de sementes de bracatinga (Mimosa scabrella Bentham) tratadas com óleos essenciais de cravo-da-índia (Syzygium aromaticum (L.) Merrill & Perry) e eucalipto citriodora (Corymbia citriodora (Hook.) KD Hill & LAS Johnson). O teste de germinação foi realizado em caixas gerbox e as avaliações foram realizadas aos 7 e 14 dias, contabilizando o número de plântulas normais, anormais, sementes mortas e duras. Para avaliar a fitotoxidez, as sementes foram tratadas em concentrações crescentes dos óleos essenciais (0, 250, 500 e 1000 ppm). Na avaliação do efeito inibitório dos óleos essenciais sobre a incidência de fungos presentes nas sementes, as mesmas foram tratadas com água destilada (controle negativo), hipoclorito de sódio 1% (controle positivo), óleo essencial de S. aromaticum 1000 ppm e óleo essencial de C. citriodora 1000 ppm. O delineamento utilizado foi inteiramente casualizado e os dados foram submetidos ao teste Z a 5% de probabilidade. Verificou-se a presença dos fungos Fusarium spp., Trichoderma spp., Pestalotiopsis spp. e Penicillium spp. nas sementes avaliadas. O óleo de cravo-da-índia apresentou potencial inibitório dos patógenos e não causou fitotoxidez nas sementes. ABSTRACT. Abstract -The objective of this study was to evaluate the health and germination of bracatinga seeds (M. scabrella Bentham) treated with essential oils of Syzygium aromaticum (L.) Merrill & Perry and Corymbia citriodora (Hook.)... Mostrar Tudo |
|
Palavras-Chave: |
Controle alternativo. |
|
Thesagro: |
Germinação; Mimosa Scabrella; Semente Florestal; Syzygium Aromaticum. |
|
Thesaurus NAL: |
Control methods; Corymbia citriodora; Seed germination. |
|
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160432/1/EmbrapaFlorestas-PFB-2023-GerminacaoSementeMimosaScabrella.pdf
|
|
Marc: |
LEADER 03416naa a2200289 a 4500
001 2160432
005 2024-01-04
008 2023 bl uuuu u00u1 u #d
022 $a1983-2605
024 7 $ahttps://doi.org/10.4336/2023.pfb.43e202002147$2DOI
100 1 $aSTANCK, J. A. T.
245 $aSanidade e germinação de sementes de Mimosa scabrella submetidas ao tratamento com óleos essenciais.$h[electronic resource]
260 $c2023
520 $aRESUMO. O objetivo desse estudo foi avaliar a sanidade e germinação de sementes de bracatinga (Mimosa scabrella Bentham) tratadas com óleos essenciais de cravo-da-índia (Syzygium aromaticum (L.) Merrill & Perry) e eucalipto citriodora (Corymbia citriodora (Hook.) KD Hill & LAS Johnson). O teste de germinação foi realizado em caixas gerbox e as avaliações foram realizadas aos 7 e 14 dias, contabilizando o número de plântulas normais, anormais, sementes mortas e duras. Para avaliar a fitotoxidez, as sementes foram tratadas em concentrações crescentes dos óleos essenciais (0, 250, 500 e 1000 ppm). Na avaliação do efeito inibitório dos óleos essenciais sobre a incidência de fungos presentes nas sementes, as mesmas foram tratadas com água destilada (controle negativo), hipoclorito de sódio 1% (controle positivo), óleo essencial de S. aromaticum 1000 ppm e óleo essencial de C. citriodora 1000 ppm. O delineamento utilizado foi inteiramente casualizado e os dados foram submetidos ao teste Z a 5% de probabilidade. Verificou-se a presença dos fungos Fusarium spp., Trichoderma spp., Pestalotiopsis spp. e Penicillium spp. nas sementes avaliadas. O óleo de cravo-da-índia apresentou potencial inibitório dos patógenos e não causou fitotoxidez nas sementes. ABSTRACT. Abstract -The objective of this study was to evaluate the health and germination of bracatinga seeds (M. scabrella Bentham) treated with essential oils of Syzygium aromaticum (L.) Merrill & Perry and Corymbia citriodora (Hook.) KD Hill & LAS Johnson. The germination test was carried out in gerbox boxes and evaluations were carried out at 7 and 14 days counting the number of normal and abnormal seedlings, dead and hard seeds. To assess phytotoxicity, seeds were treated with increasing concentration of essential oils (0, 250, 500 and 1000 ppm). To evaluate the inhibitory effect of essential oils on the incidence of microorganisms present in the seeds, they were treated with distilled water (negative control), sodium hypochlorite 1% (positive control), essential oil of S. aromaticum 1000 ppm and essential oil of C. citriodora 1000 ppm. The design was completely randomized, and the data were compared using the Z test at 5% probability. We verified the occurrence ofFusarium spp., Trichoderma spp., Pestalotiopsis spp. and Penicillium spp. pathogens at evaluated seeds. S. aromaticumessential oil showed potential to inhibit pathogens and did not cause phytotoxicity in M. scabrella seeds.
650 $aControl methods
650 $aCorymbia citriodora
650 $aSeed germination
650 $aGerminação
650 $aMimosa Scabrella
650 $aSemente Florestal
650 $aSyzygium Aromaticum
653 $aControle alternativo
700 1 $aSANTOS, G. C. dos
700 1 $aTOLENTINO JÚNIOR, J. B.
700 1 $aGRAF JÚNIOR, A. L.
700 1 $aITAKO, A. T.
773 $tPesquisa Florestal Brasileira$gv. 43, e202002147, p. 1-7, 2023
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Florestas (CNPF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
